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sheep polyclonal igg  (R&D Systems)


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    R&D Systems sheep polyclonal igg
    Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep <t>polyclonal</t> IgG <t>(AF5195,</t> R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.
    Sheep Polyclonal Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+polyclonal+igg+af5195/Mouse+IL-34+Antibody/pmc07580706-33-40-44
    Average 94 stars, based on 21 article reviews
    sheep polyclonal igg - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration"

    Article Title: Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.579000

    Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.
    Figure Legend Snippet: Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.

    Techniques Used: Activation Assay, Inhibition, Western Blot, Phospho-proteomics, Comparison, Control

    Intracerebral IL-34 antibody administration resulted in reduced microglia proliferation in ME7 prion mice. (A) Mice infected with prion disease received a single intracerebral injection of mouse- or human-specific anti-IL-34 (sheep polyclonal IgG) and brains were analyzed 1 week later. (B) Histological analysis of BrdU/Iba1-positive microglial cells in the cortex showed a reduction after treatment with a mouse-, but not with a human-specific antibody. Scale bar 100 μm. Naïve n = 6, ME7 n = 12, ME7 + isotype n = 10, ME7 + anti-mIL-34 n = 8, ME7 + anti-hIL-34 n = 7, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Intracerebral IL-34 antibody administration resulted in reduced microglia proliferation in ME7 prion mice. (A) Mice infected with prion disease received a single intracerebral injection of mouse- or human-specific anti-IL-34 (sheep polyclonal IgG) and brains were analyzed 1 week later. (B) Histological analysis of BrdU/Iba1-positive microglial cells in the cortex showed a reduction after treatment with a mouse-, but not with a human-specific antibody. Scale bar 100 μm. Naïve n = 6, ME7 n = 12, ME7 + isotype n = 10, ME7 + anti-mIL-34 n = 8, ME7 + anti-hIL-34 n = 7, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Infection, Injection, Comparison

    Related Articles

    Injection:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.

    Activation Assay:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.

    Inhibition:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.

    Western Blot:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.

    Phospho-proteomics:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.

    Comparison:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.

    Control:

    Article Title: Inhibition of IL34 unveils tissue-selectivity and is sufficient to reduce microglial proliferation in chronic neurodegeneration
    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, −2.0 mm; lateral, ±1.7 mm; depth, −1.6 mm, 12 weeks after induction of prion disease.



    Similar Products

    sheep polyclonal igg  (R&D Systems)
    94
    R&D Systems sheep polyclonal igg
    Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep <t>polyclonal</t> IgG <t>(AF5195,</t> R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.
    Sheep Polyclonal Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+polyclonal+igg+af5195/Mouse+IL-34+Antibody/pmc07580706-33-40-44
    Average 94 stars, based on 1 article reviews
    sheep polyclonal igg - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    sheep polyclonal igg af5195  (R&D Systems)
    94
    R&D Systems sheep polyclonal igg af5195
    Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG <t>(AF5195,</t> R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.
    Sheep Polyclonal Igg Af5195, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+polyclonal+igg+af5195/Mouse+IL-34+Antibody/pmc07580706-46-16-22
    Average 94 stars, based on 1 article reviews
    sheep polyclonal igg af5195 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.

    Journal: Frontiers in Immunology

    Article Title: Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration

    doi: 10.3389/fimmu.2020.579000

    Figure Lengend Snippet: Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.

    Article Snippet: Mouse myelogenous leukemia (M-NFS-60) cells were CSF-1 (R&D systems, 216-MC/CF) starved for 24 h. In white clear bottom 96-well-plates 10 μL IL-34 antibody (mouse monoclonal IgG2A (v1.1 manufactured by Genscript, ( , )), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems) and 10 μL IL-34 stimulus (R&D systems, 5195-ML-CF) were incubated at 37°C for 30 min before 80 μL M-NFS-60 cells (10 3 cells/well) were added.

    Techniques: Activation Assay, Inhibition, Western Blot, Phospho-proteomics, Comparison, Control

    Intracerebral IL-34 antibody administration resulted in reduced microglia proliferation in ME7 prion mice. (A) Mice infected with prion disease received a single intracerebral injection of mouse- or human-specific anti-IL-34 (sheep polyclonal IgG) and brains were analyzed 1 week later. (B) Histological analysis of BrdU/Iba1-positive microglial cells in the cortex showed a reduction after treatment with a mouse-, but not with a human-specific antibody. Scale bar 100 μm. Naïve n = 6, ME7 n = 12, ME7 + isotype n = 10, ME7 + anti-mIL-34 n = 8, ME7 + anti-hIL-34 n = 7, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration

    doi: 10.3389/fimmu.2020.579000

    Figure Lengend Snippet: Intracerebral IL-34 antibody administration resulted in reduced microglia proliferation in ME7 prion mice. (A) Mice infected with prion disease received a single intracerebral injection of mouse- or human-specific anti-IL-34 (sheep polyclonal IgG) and brains were analyzed 1 week later. (B) Histological analysis of BrdU/Iba1-positive microglial cells in the cortex showed a reduction after treatment with a mouse-, but not with a human-specific antibody. Scale bar 100 μm. Naïve n = 6, ME7 n = 12, ME7 + isotype n = 10, ME7 + anti-mIL-34 n = 8, ME7 + anti-hIL-34 n = 7, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Mouse myelogenous leukemia (M-NFS-60) cells were CSF-1 (R&D systems, 216-MC/CF) starved for 24 h. In white clear bottom 96-well-plates 10 μL IL-34 antibody (mouse monoclonal IgG2A (v1.1 manufactured by Genscript, ( , )), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems) and 10 μL IL-34 stimulus (R&D systems, 5195-ML-CF) were incubated at 37°C for 30 min before 80 μL M-NFS-60 cells (10 3 cells/well) were added.

    Techniques: Infection, Injection, Comparison

    Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.

    Journal: Frontiers in Immunology

    Article Title: Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration

    doi: 10.3389/fimmu.2020.579000

    Figure Lengend Snippet: Activation of the CSF1R signaling pathway by IL-34 and CSF-1 and inhibition of IL-34-mediated cell growth using IL-34 neutralizing antibodies. (A) N13 microglia cell line was stimulated with IL-34 (50 or 100 ng/mL), CSF-1 (50 or 100 ng/mL) or LPS (1 μg/mL) for 5 or 10 min. Cell lysates were subjected to Western blotting which showed increased phosphorylation of CSF1R and downstream ERK1/2 and AKT after IL-34 and CSF-1 stimulation. (B) IL-34 neutralizing antibodies used in this study (mouse monoclonal IgG2A [v1.1, ), rat monoclonal IgG2A (MAB5195, R&D Systems) and sheep polyclonal IgG (AF5195, R&D Systems)] prevented IL-34-dependent cell growth of M-NFS-60 in a similar nanomolar range. n = 3, data shown represent mean ± SEM, two-way ANOVA followed by Tukey's multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, comparisons are stimulations vs. unstimulated control (C) for each time point.

    Article Snippet: For acute treatment in ME7 prion mice, 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195, or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus, coordinates from bregma: anteroposterior, ?2.0 mm; lateral, ±1.7 mm; depth, ?1.6 mm, 12 weeks after induction of prion disease.

    Techniques: Activation Assay, Inhibition, Western Blot, Phospho-proteomics, Comparison, Control